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KMID : 0368420060490040303
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Journal of Plant Biology
2006 Volume.49 No. 4 p.303 ~ p.308
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A 273-bp promoter region is responsible for circadian regulation ofS-adenosylmethionine decarboxylase gene expression in carnation
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Kim Young-Jin
Kim Yong-Bum
Lee Sun-Hi
Park Ky-Young
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Abstract
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S-Adenosylmethionine decarboxylase (SAMDC) activity and mRNA amount increase in a light-dependent manner, and the accumulation ofSAMDC transcripts might be partially regulated by a circadian clock in tritordeum andPharbitis nil. However, no research has been reported on the circadian regulation of SAMDC activity and corresponding gene expression. Here, we determined that SAMDC activity and its mRNA accumulation changed diurnally in carnation leaves. To identify the promoter region responsible for this rhythmicity, we performed northern blot analysis using aGUS cDNA probe in transgenic tobacco plants. The CCS gene was under the control of serial deletions of the 5′-flanking region of a genomic clone corresponding toCSDC9 (gCSDC9). The region between -754 and -274, which negatively regulated the accumulation ofGUS mRNA, had two HD-Zip-2 binding sequences (TAATAATTA) that were found by PLACE analysis of the promoter region. Another region at 273 bp upstream from the transcription initiation site was sufficient for diurnal expression ofGUS, and contained the putative sites responsible for diurnal expression, i.e., the GT-1 consensus sequence (GGTAAT) and a sequence necessary for this circadian expression (CAACTTCATC).
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KEYWORD
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S-adenosylmethionine decarboxylase, carnation, circadian clock, cis-element, promoter, transcriptional regulation
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